The interaction between MYB proteins and their target DNA

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4/28/2005 · Here, we report a minimally invasive optical technique that uses 405‐nm laser light to photoactivate a variant of green fluorescent protein (PAGFP). Single cells and small groups of cells (n < 10) are successfully marked, from a region of cells injected and electroporated with PAGFP, in both whole chick embryo explants and in ovo. more


Coordinated binding of NF-κB family members in the

Therefore, short H-NS binding regions tend to behave in a manner typical of canonical TFs, where the protein binds upstream of the gene whose expression it regulates. On the other hand, longer binding regions wrap large segments of the chromosome, encompassing both genes and intergenic regions. Variable structures of Fis–DNA complexes more


(PDF) Advances in single-cell RNA Sequencing and its

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Photoactivatable green fluorescent protein as a single

Single cells were photoactivated in a number of embryos (the number of embryos analyzed for each construct is shown in the graph), which were re‐incubated and surveyed at t = 24 and 48 hr. Kaede is the only PAFP that was unable to be found at t = 24hr and the points marked are t = 15 hr. In a similar manner, PAGFP photoactivated cells more


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1/1/2012 · By contrast, single-repeat MYB proteins, which only possess one MYB DNA-binding repeat, bind DNA in a different manner than R1R2R3 and R2R3–MYB proteins . This first became clear when S. cerevisiae Rap1 was found to contain two MYB repeats in its MYB–DNA-binding domain and its orthologous MYB counterpart, Homo sapien RAP1, only possessed more


Computational and analytical challenges in single-cell

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Single-Molecule Tracking in Living Cells Using Single

Moreover, ERα occupancy at the −168 kb region occurred in a cyclical manner with peaks at 30 and 60 min or later after the addition of E2, a timing very similar to that observed for ERα binding to the two major ERα binding sites associated with the TFF1 gene (Fig. 2 D) (32–34). more


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Gene-Specific Patterns of Coregulator Requirements by

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Chromatin accessibility: a window into the genome

1/28/2015 · Technological advances in single-cell RNA sequencing (scRNA-seq) now enable the transcriptomes of large numbers of individual cells to be assayed in an unbiased manner. more


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Differential response of carbon fluxes to climate in three

10/7/2011 · Therefore, this pancRNA could influence the epigenetic processes as a ssRNA. ssRNA-induced DNA methylation changes have been reported for the rat Sphk1/Khps1 locus (28, 29), and thus interaction of a promoter with a single-stranded pancRNA appears to be one type of general mechanism regulating epigenetic status in a sequence-specific manner. more


Chromatin three-dimensional interactions mediate genetic

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Large Scale Loss of Data in Low-Diversity Illumina

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Single-stranded Noncoding RNAs Mediate Local Epigenetic

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